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Image Search Results
Journal: Viruses
Article Title: Genomic Characterization and gE/gI-Deleted Strain Construction of Novel PRV Variants Isolated in Central China
doi: 10.3390/v15061237
Figure Lengend Snippet: Construction and identification of the mutant virus SX1911-ΔgE/gI. ( A ) Strategy for constructing SX1911-ΔgE/gI using the CRISPR/Cas9 and LoxP systems. Two sgRNAs were designed to guide Cas9 to delete the gE and gI genes, and GFP was used for both positive and negative screening of mutant virus production. ( B ) Identification of SX1911-ΔgE/gI via IFA and PCR targeting the gE gene. ( C ) Multistep growth curve of SX1911 and SX1911-ΔgE/gI in Vero cells. ( D ) Plaque sizes of SX1911 and SX1911-ΔgE/gI in Vero cells. Data are presented as the mean ± SD, and an asterisk indicates a significant difference between SX1911 and SX1911-ΔgE/gI. ***: p < 0.001.
Article Snippet: sgRNAs targeting the gE and gI genes were designed using an online
Techniques: Mutagenesis, Virus, CRISPR
Journal: Frontiers in Genetics
Article Title: Analysis of the type II-A CRISPR-Cas system of Streptococcus agalactiae reveals distinctive features according to genetic lineages
doi: 10.3389/fgene.2015.00214
Figure Lengend Snippet: Genetic organization of the CRISPR1- cas locus in Streptococcus agalactiae . cas genes and core genes are shown as black arrows and blue arrows, respectively. The leader sequence is located between the cas gene cluster and the CRISPR array (white box; L) while the trailer sequence is located downstream of the array (white box; T). The direct repeats (DR) are shown as black diamonds and the terminal repeat, which differs from the consensus DR, is shown as a white diamond. Spacers are shown as colored rectangles and unique spacers are represented by unique colors. Below the CRISPR array, the sequence of the first two repeat-spacer units is shown with the DRs in black characters and the spacers in color characters.
Article Snippet: In a second step, CRISPR1 array structure was determined anew using a
Techniques: Sequencing, CRISPR